1Department of Biochemistry, Federal University of Technology, Owerri, Imo State, Nigeria
2Department of Haematology, Abia State University, Uturu, Nigeria
3Department of Obstetrics and Gynecology, Abia State University, Uturu, Nigeria
4Department of Surgery, Abia State University, Teaching Hospital, Aba, Nigeria
5Department of Surgery, Abia State University, Uturu, Nigeria
6Department of Internal Medicine, University of Port-Harcourt Teaching Hospital, Rivers state, Nigeria
7Department of Community Medicine, Abia State University Teaching Hospital, Aba, Nigeria
8Department of Haematology and Blood Transfusion, University of Port Harcourt, Nigeria
9Department of Haematology, Federal Medical Centre, Umuahia, Abia State, Nigeria
Background: In order to learn more about the extent to which Corchorus olitorius (C. olitorius) leaves may affect experimentally-induced testicular toxicity in light of the fact that the literature in this area is quite scanty, this study investigated the mitigative effects of C. olitorius leaf extract against infertility induced by potassium bromate on male Wistar rats.
Methodology: C. olitorius was extracted with soxhlet extractor with ethanol as the solvent. Twenty-four adult male Wistar rats were acclimatized under laboratory conditions and were randomly grouped into A, B, C and D. Group A was given distilled water orally. Animals in groups B, C and D were administered 100 mg/kg body weight of potassium bromate, but groups C and D were also treated with 100 and 200 mg/kg body weight of C. olitorius respectively. Both potassium bromate and C. olitorius were freshly prepared on daily basis and administered to rats by oral gavage. After 28 days of treatment, the animals were sacrificed under mild diethyl ether anaesthetization 24 hours after cessation of last treatment. The testes were removed homogenized in the ice cold 0.25M sucrose solution. The homogenates were centrifuged at 5000 ×g for 10 minutes in a refrigerated centrifuge. The supernatant was collected and stored frozen for further analysis. The parameters were measured using standard methods.
Results: When compared to animals in the control group, animals intoxicated with KBrO3 had lower testicular levels of total cholesterol, total protein, glycogen, sialic acid, GSH, as well as ALP, SOD, and CAT activity. Additionally, it was shown that as compared to the animals in the control group, KBrO3 boosted the testicular MDA and ACP's activity. However, C. olitorius treatment of intoxicated rats reduced these alterations in a dose-dependent manner.
Conclusion: Potassium bromate induced testicular toxicity by unhinging the biochemical indices and increasing lipid peroxidation in the testes of exposed animals. This effect was mitigated by coadministration with C. olitorius leaf extract. Therefore, it is advised that clinical trials involving human volunteers be conducted to further explore these findings.
Keywords: Corchorus olitorius; Oxidative Stress; Potassium Bromate; Testicular Toxicity
AI Airaodion.,et al. Mitigation of Experimentally-Induced Testicular Toxicity by Corchorus olitorius Leaves. EC Pharmacology and Toxicology 11.2 (2023): 14-24.
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